Spectinomycin Rapid Test Strip In Dairy Milk Products

Brief:

The Spectinomycin rapid test strip is used for testing Spectinomycin residue in milk qualitatively. The whole test only need 8min, east to operate with high sensitivity.

Detection limit: 5~8ng/ml (ppb)

Principle:

The Spectinomycin rapid test strip is based on competitive inhibition immuno-chromatographic principle. In the flow process, Spectinomycin in the sample combined with Spectinomycin specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Spectinomycin-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the sample has no Spectinomycin residue or concentration lower than detection limit, T line is darker then C line; when the concentration is equal to or higher than detection limit, T line is lighter than C line or T line has no color or T line has same color with C line. No matter whether there is Spectinomycin residue in sample, C line will appear, it means the test is valid.

Operation procedures:

1 Read the instruction carefully before use. Return test strips and sample into room temperature.

2 Take bottles needed from the kit package, take out required microwells, making proper marks, Please use it in 1 hour. Seal the cap of the bottles, avoid moisture.

3 Use Micro-pipette to take 200ul of the samples into the microwells, then repeatedly absorb for 5 times, mix the sample with the reagent in the microwells completely until no solid judged by eyes (this is a very important step).

4 Incubate at 40±2℃for 3 min. Then insert the strip into micro-well, put the end with MAX down into the liquid.

5 Insert the strip into the liquid for 5min, then read the result.

Test Result Interpretation:

Results picture show:

Precautions:

1) The test strip is used for once at room temperature, do not use test strip out of date.

2) Cover the bottle immediately after taking out the reagent. If can’t use 8 micro-wells for one time, cover the micro-wells immediately and put back to the bottle, store in seal.

3) Do not touch the white membrane surface in the middle of test strip, avoid sunlight and fan blowing directly.

4) Milk samples should be fresh and no polluted, for sample store in cold and frozen should return to room temperature before testing.

5) Use the test strips testing again for positive results.

6) Please contact the supplier for any questions.

7) The result is for reference. To confirm, please refer national standard method.

Specificity:

This product has no cross-reaction with Quinolones, Sulfonamides and Chloramphenicol etc.

Storage and expiry date:

Storage: Store at 2-8 ℃ in sealed, dry, and dark place,no frozen.
Expiry date: 12 months; date of production is on box.

Detection limit: 5~8ng/ml (ppb)